Advances in spectroscopy for lasers and sensing by Baldassare Di Bartolo; Ottavio Forte; International School

By Baldassare Di Bartolo; Ottavio Forte; International School of Atomic and Molecular Spectroscopy.; North Atlantic Treaty Organization

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Spectroscopy of Photoni Atoms 11 7. Specific Detection of Protein 12 Up until now we have considered protein adsorbed on a silica surface. There are a number of ways in which this can be facilitated. On means is to functionalize the silica surface with an agent such as 3-aminopropyltriethoxysilane (APTES). This compound reacts with the silanol groups on the silica surface and at biological pH leaves an exposed NH3+ rug. This is ideal for a protein that acquires a negative charge at this pH; the charge attraction holds the protein in place.

These shift are plotted against each other in Fig. 5. In a separate experiment, we added an infinite layer thickness perturbation by increasing the refractive index of the surrounding medium (water) by adding NaCl. In each case the shifts of resonances centered about two wavelengths, λ1 = 760 nm and λ2 = 1310 nm were measured. These shift are plotted against each other in Fig. 4. 04, which compares well with the t / L <<1 limit for Eqn. 00. 54 whereas the t / L >> 1 limit of Eqn. 58. This good agreement tests the wavelength dependence of our shift equation and allows the technique to be used as a nanoscopic ruler.

Fig. 12B shows this differential signal. Here we see the real time discrimination of one letter of DNA with a very large signal-to-noise ratio, 54! 16 Stephen Arnold and Ophir Gaathon 9. Future Directions By reducing the microsphere radius our sensitivity will increase4 allowing us to see smaller and smaller fractions of a monolayer, until we may ultimately reach a single bio-particle. At this point it may be possible to count label-free molecules one at a time rather than wait for a full monolayer to form.

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